The Effect of Ethanolic Extract of Purple Yam Tuber (Dioscorea alata L.) on Bone Calcium Levels in Ovariectomized Rat

Abstract

This study is on the effects of the ethanolic extract of purple yam tuber (Dioscoreaalata L.) on the bone calcium levels in ovariectomized (ovx) rat models of osteoporosis. Forty-five of 8-weeks-old female Sprague–Dawley rats were randomly assigned to six groups as followed: sham-operated, OVX, OVX-Estradiol, OVX-EDA 116 mg/kg BW, OVX-EDA 232 mg/kg BW, OVX-EDA 463 mg/kg BW for 30 days. The administration of EDA and estradiol was given orally using a stomach tube.It is on the 31st day since the bones of femur and Tibia rat were isolated. The isolated bones were analyzed using wet destruction and the bone calcium level was measured using the AAS. The bone calcium level were tested statistically using one way ANOVA.The average of bone calcium levels for each group: for the normal group is 18,30 + 3,17%, OVX 16,44 + 3,93%, OVX-Estradiol 16,59 + 2,63%, and for the group of OVX-EDA dose 116; 232; 463 are 15,89 + 1,66%, 15,96 + 2,61%, and 14,91 + 2,31% respectively. The conclusion is that the treatment of purple yam tuber (DioscoreaalataL.) with the different dose of 116; 232; and 463 mg/kg BW for 30 days cannot increase the bone calcium level in the ovariectomized rats. According to the statistical data there is no significant differences among the treatment groups.

Keywords: Calcium, .Dioscorea alata, Extract