Correlation between Leukocytospermia Detected by Cytochemical Peroxidase Staining and Chlamydia Trachomatis Infection in Iraqi infertile Males

Abstract

Background and objective: To detect pus cells in semen by cytochemical peroxidase staining method and to study whether there is a difference in its distribution among infertile males with positive, negative titer of antichlamydial immunoglobulins compared with normal fertile males  Patients and Methods: Semen samples were collected from 144 infertile male, 35 normal fertile.  Seminal plasma was used for detection of chlamydia antibodies by Microimmunofluorescence test (MlF) using species-specific antigens (genital serotypes D-K)Results:Only 1/144(0.69%) was IgM positive at a titer (1:16) in secondary infertile. Only 3/144(2.08%) primary infertile males were IgG positive, the frequent titer was (1:64). Only 64/144 (44.44%) infertile were IgA positive. IgA was detected in (65.62%) among primary infertile at a titer (1:64)  . Only 40/144 infertile  (27.77%) were IgA and IgG positive .IgA and IgG were detected together in (52.5%) among secondary infertile males at a titer (1:64) and at a titer (1:8) in (12.5%) among primary infertiles. No classes of immunoglobulines were detected at any titer in seminal plasma among normal fertile males.Out of 144 infertile males 93(64.58%) with history of urogenital tract infection (UGI)  . The majority of males with history of UGI were primary infertile with positive titer in seminal plasma. Only 4/108 (3.7%) azoospermic males with   positive titer in seminal plasma have previous history of   (UGI) .Peroxidase positive leukocytes with less than 1x10⁶ cell/ml of semen were detected in 108/179(60.33%) and  (32.96%) were infertile with positive titer .Peroxidase positive leukocytes ≥ 1x106 cell/ml of semen   detected in (24.58%) . The incidence of this count was high among infertile males with positive titer (16.2%) . The incidence of peroxidase positive leukocytes at the count more than 4x106 cell/ml of semen was high among infertile males with positive titer.In conclusion: There was no relationship between count of pus cells and antichlamydial antibodies in seminal plasma of infertile males. Antichlamydial immunoglobulins were detected in (32.96%) among infertile males with positive titer in seminal plasma and pus cell ˂1x10⁶ cell/ml of semen.  The cytochemical peroxidase method is cheap, fast and easily to perform and reliable to identifies granulocytes, which is the most prevalent WBC type in semen.

Keywords: Infertility, Antichlamydial immunoglobulins, pyospermia, peroxidase