Protective effect of chamomile recutita flowers extract against Urinary tract infection induced by Pseudomonas aeruginosa in Experimental mice models

Abstract

This study was conducted to investigate the protective role of ethanolic extract of chamomile recutita flowers induced UTI in the mice by Pseudomonas aeruginosa,the flowers extracted by preparing ethanol extract .10⁸ cfu /ml of Pseudomonas aeruginosa was used to induce UTI by intra-urinary bladder injection . Thirty mice were divided into three groups .Group I: negative control (normal). Group II: positive control, mice were infected by injection of Pseudomonas aeruginosa for 2, 7, 15, 21 and 30 days and left without treatment. Group III : mice infected and treated with ethanolic extract of of Chamomile (Matricaria recutica ) flowers orally by stomach tube daily after 48 h of  infection for  15 , 21 and 30 days at dose 300 mg/kg-BW. At the end of experimental period ,blood samples were taken from mice and measured some biochemical parameters such as serum Creatinine , Serum uric acid ,serum glucose and Serum total proteins .animals of all groups were killed to examine histopathological changes in urinary bladder and kidney .The result showed significant increased (P< 0.05) in serum creatinine level and serum uric acid in infected groups as compared with control .The treated group showed significant decreased (P< 0.05)  in serum creatinine level and serum uric acid level while no significant change in serum glucose level and serum total protein in infected and treated groups. Histological sections of kidney appeared damage, necrosis of renal tubule and inflammatory cell infiltration in infected groups while animals treated with ethanolic extract of Chamomile recutica flowers showed look -like normal appearance in kidney. It could be concluded that Chamomile recutica flower extract has potent protective role against UTI and appears by correction between biochemical parameters and histological studies.