Determination the genotyping diversity between biofilm forming and collagenase producing Pseudomonas aeruginosa strains

Abstract

A total of 82 Pseudomonas aeruginosa strains isolated from four hospitals in Baghdad. The isolates were studied by repetitive element based PCR (rep-PCR) using BOX primer.Methode: Biofilm determination method was used to screen the 82 isolates of forming biofilm .Collagenase production assay was used to screen the 28 isolates that were strong biofilm formers Collagenase production assay was used to screen the 28 isolates that were strong biofilm formers.Results: collagenase production increases when bacteria switch from a planktonic to biofilm phenotype. This indicates that biofilms and collagenase are more virulent and have a greater ability to cause tissue destruction . The REP-PCR analysis using BOX-primer, showed a clusters genetic relatedness among the isolates. The isolates were grouped according to the REP-PCR in 9 different genotypes, named cluster 1 to 3 which included  C1, C2 ,C3 with relatedness  :8 (80%), 8 (86%) ,3 (80%) respectively . A19 and A20 both of them were not included in any cluster , they have 78% similarity .The REP-PCR analysis showed that the genotypic relatedness is consistently high between the 8 producer isolates and non producer isolates (13),showed similarity reached 86% between collagenase and biofilm producers .

Keywords: Genotyge ,rep-PCR,Collagenase,Biofilm,Pseudomonas aeruginosa,BOX primer