The Analysis of Tempe Consumption Effects to Obesity in Adolescents through DNA Methylation

Abstract

Obesity is a health problem worldwide, even WHO states that obesity is already a global epidemic, so obesity is a health problem that must be addressed immediately. Obesity is a nutritional problem with an increasing prevalence and sufferers not only individuals aged but also school-age children and children aged under five. This study has the objectives of 1) Knowing the level of consumption of tempe in adolescents, 2) Knowing the influence of tempe consumption on obesity in adolescents through DNA methylation.This obesity study was taken from obese population in the 2nd grade of Kediri Junior High School, while the normal weight sample was equal to the number of Obesity samples and taken from the same grade students by Simple Random Sampling. The results of the laboratory analysis of this study used primary data to amplify the leptin promoter gene region. Based on the analysis results, there is no change in the methylation area. There are samples that have mutations or even large amounts of nucleotide changes in areas that are not predictive sites of mutilation. This study has the following conclusions: 1) The samples that have mutations or even large amounts of nucleotide changes in areas that are not methylated prediction sites, 2) Changes in this promoter area are likely to affect gene expression, 3) In the sample Obesity 100% mutation, while the sample Non Obesitas 50% have mutations, 4) After re-checking, these mutations or nucleotide changes in some samples occur in areas that are transcription factors associated with leptin production and obesity conditions, 5) The teens of Junior High School in Kediri is not found methylation in genes related to leptin production, but mutation is found, 6) In children, obesity has 2x mutation is greater than non obesity child.

Keywords: Obesity, DNA methylation, mutation, Simple Random Sampling.