The Content of Total Phenols and Antioxidant Activity Three Types Sea Algae Taken at the North Sulawesi Waters

Abstract

This research aims to study the total content of phenolic and antioxidant activity in fractions of hexane, ethyl acetate and water at sea Caulerpa sertularoides sea algae, Laurencia tronoi and Padina australis. The content of total phenols were measured using the method Fholin-Chiocalteau, while the measurement of antioxidant activity using free radical scavengers 1,1-diphenl-2-picryhidrazyl (DPPH), reducing power, and ferrous ion chelating (FIC). Mass fraction of water is highest on Caulerpa sertularoides fraction (12.3 g), and the lowest Padina australis hexane fraction (0.44 g). Caulerpa sertularoides ethyl acetate fraction was found have the highest content of total phenols 123.87 ± 2.67 mg GAE / g extract and reducing the highest power is 36.45 ± 0.27 mg GAE / g, whereas the water fraction of Laurencia tronoi have the lowest total phenol content that is 5,92 mg GAE / g extract and lowest FRAP is 1.33 mg GAE / g extract. Highest activity of free radicals found in the hexane fraction tronoi Laurencia is 82.62 ± 1.54%, the highest FIC at a concentration of 1000 ppm, 1500 ppm and 2000 ppm was found in the water fraction Caulerpa sertularoides respectively 93.76 ± 0.4% ; 94.65 ± 0.74% and 96.50 ± 0.26%. FIC value of three factions in the three types of marine algae increased with increasing concentration of the sample.

Keywords: marine algae; total phenol; antioxidant activity