Testing of Hypocholesterolemic Effects on Instant Ledok In Vivo

Abstract

Ledok is a traditional food in Nusa Penida, in form of porridge made from corn, cassava, beans, green leaves vegetables and seasoning without rice. Ledok instant is development of ledok which requires preparation times around five minutes. Ledok instant has the potentials to be developed as a functional food, because it contents sufficient nutrients and bioactive component which is dietary fiber. The objectives of this research is to discover the hypocholesterolemic effect of instant ledok in vivo. The research begins with producing instant ledok added with purple sweet potato, production of standard feed and treatment feed (standard feed + instant ledok) and continued with bioassay testing using experiment rats. The experiment is conducted by Completely Randomized Design. There are four treatments i.e : P0 (rat group without hypercholesterol + standard feed = negative control), P1 (rat group with hypercholesterol + standard feed = positive control), P2 (rat group with hypercholesterol + standard feed + instan ledok 15 %) and P3 (rat group with hypercholesterol + instantledokof 30 %). Every group consists of seven rats. Analysis are taken before (pretest) and after (post test) on cholesteroltotal,cholesterol HDL, cholesterol LDL, triglyceride of rat blood and rat body weight. The feed treatment is given for 28 days. The research shows that there is a lowering of  cholesterol total level, triglyceride and cholesterol LDL of rats blood. In the other hand there is raise in rats blood cholesterol HDL level take place by giving mix of standard feed and instant ledok. Treatment of ledok instant by 30 % can lower cholesterol total level, triglyceride and cholesterol LDL in the following order : 42.48 %, 25.29 % and 53.04 % from pretest level which are : 208.15 mg/dl, 136.50 mg/dl and 74.07 mg/dl. Meanwhile cholesterol HDL level of rat blood has a raise of 150 % from pretest level 26.40 mg/dl. Rats body weight during the experiment raises about 14.5 % - 16.6 % from the original weight 218.1 g – 226.1 g.

Keywords : instant ledok, hypocholesterolemic, dietary fiber, cholesterol, triglyceride