Effects of Germination Parts of Seed Legumes on Histamine Levels in Rats

Abstract

Diamine oxidase (DAO) is a major enzyme in the breakdown of dietary histamine. Histamine intolerance is caused by an imbalance of histamine scavenging mechanisms, which can be treated with small-histamine diet. The relevance of dietary supplements containing external DAO has lately grown especially that produced from low-cost sources to aid in the intestinal hydrolysis of histamine. As a result, this study aimed to test the sprouted sections of fenugreek and beans for histamine breakdown to determine their availability as nutritional supplements used to get rid of histamine and limit the harm caused by excessive concentrations which can lead to death. The impact of adding 10% lyophilised sprouted bean, 10% lyophilised sprouted fenugreek, and blends of them at 5% of each, on mice administrated orally histamine solution (200 mg/kg bw) was investigated. When compared to the positive control, the diet contain lyophilised legume sprouts (10% fenugreek, 10% bean, and their mixture at 5% from each of them) it found that histamine reduced by 29, 21, and 36 %, while glutathione increased by 129, 96, and 150 %, respectively. In addition, all liver function as well as malondialdehyde decreases, while the liver antioxidant enzymes were enhanced. Finally, the findings show that legume sprouts (fenugreek and beans) can be used to make functional enzymatic supplements that can help alleviate histamine intolerance symptoms.

Keywords: Histamine, Histamine Intolerance, Diamine Oxidase (DAO) Enzyme, Legumes, Sprouted Fenugreek and Beans

DOI: 10.7176/FSQM/116-05

Publication date:June 30^th 2022