Antioxidant Activity Against Methanol Extraction of Eucheuma cotonii and E. spinosum Collected From North Sulawesi Waters, Indonesia

Abstract

Eucheuma cotonii and E. spinosum cultivated in Arakan waters, North Sulawesi, Indonesia, were tested the antioxidant activity of fresh and dry samples through maceration in 60%, 70%, and 80% methanol solvent.  The tested antioxidants were total phenol, DPPH, FRAP, and total carotene, respectively.

Fresh E. spinosum dissolved in 60% methanol had the highest total phenol, 5.87±0.15 mg GAE/g, the highest DPPH, 75.27 ± 0.29 %, the highest FRAP, 44.52 ± 1.27 mg/l, respectively. The highest total carotene, 9.40±0.35 µg/g, was recorded in fresh E. cotonii, followed by fresh E. spinosum, 8.73±0.23 µg/g, which were also dissolved in 60% methanol. Therefore, the best antioxidant activity was found in fresh E. spinosum macerated in 60% methanol.

Keywords: Alga, antioxidant activity, phenol, DPPH, FRAP, carotene